Fluorescent labeling analysis and electron probe microanalysis for alveolar ridge augmentation using calcium phosphate cement.

نویسندگان

  • Kenji Fujikawa
  • Akiyoshi Sugawara
  • Kaoru Kusama
  • Minoru Nishiyama
  • Seidai Murai
  • Shozo Takagi
  • Laurence C Chow
چکیده

Our previous histopathological study showed that the augmentation block, prepared from a calcium phosphate cement (CPC) mixed with H2O at powder to liquid ratio of 5 g/mL, placed on the alveolar bone ridge, was gradually replaced by natural bone. In the present study, fluorescent labeling analysis (FLA) and electron probe microanalysis (EPMA) were performed on the same surgical site of the above histopathological study. Fluorescent labeling agents, that would be incorporated into newly formed mineralized tissues, were injected into dogs intramuscularly twice a week during the 3 week period that ended 1 week before sacrifice. The specimens obtained from the block were subjected to FLA for assessing the extent of new bone formation and to EPMA for measuring the elemental (Ca, P, Mg) distributions. FLA results showed the presence of newly formed bone at 1 month after surgery. EPMA results showed that the elemental distributions in the augmentation site were similar to those of the residual bone area at 6 months after surgery. FLA and EPMA examinations also indicated that the implants were surrounded and fixed by natural bone chronologically. A CPC augmentation block is clearly useful for alveolar ridge augmentation and osteointegrated implant fixation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Lateral alveolar ridge augmentation procedure using subperiosteal tunneling technique: a pilot study

Background: In this research article, we evaluate the use of sub-periosteal tunneling (tunnel technique) combined with alloplastic in situ hardening biphasic calcium phosphate (BCP, a compound of β-tricalcium phosphate and hydroxyapatite) bone graft for lateral augmentation of a deficient alveolar ridge. Methods: A total of 9 patients with deficient mandibular alveolar ridges were included in t...

متن کامل

Alveolar ridge augmentation using various bone substitutes--a web form of titanium fibers promotes rapid bone development.

Alveolar ridge augmentation with beta-tricalcium phosphate (beta-TCP) granules, calcium phosphate cement (CPC) powder and web form of titanium fibers (TW) added to Platelet-Rich-Plasma (PRP) was histologically observed for 5 months by the experiments using the maxilla of rabbits. As a result, TW was thus found to promote the rapid bone development within a period of less than 5 months, because ...

متن کامل

A Two-step Method for the Preparation of Implant Recipient Site in Severe Atrophic Maxilla: A Case Report of the Alveolar Ridge Split Technique Followed by Bone Expansion

Augmentation of deficient and atrophic alveolar ridges is an important aspect of dental implant therapy with the goal of providing a functional restoration in harmony with adjacent natural dentition. Bone splitting technique is considered a distinguished augmentation method for treatment of deficient alveolar ridges. According to this procedure, the compromised alveolar ridge is opened from the...

متن کامل

Injectable bone substitute to preserve alveolar ridge resorption after tooth extraction: a study in dog.

The aim of the present study was to assess the efficacy of a ready-to-use injectable bone substitute on the prevention of alveolar ridge resorption after tooth extraction. Maxillary and mandibular premolars were extracted from 3 Beagle dogs with preservation of alveolar bone. Thereafter, distal sockets were filled with an injectable bone substitute (IBS), obtained by combining a polymer solutio...

متن کامل

Investigation of Macroporous Calcium Phosphate Cement Obtained by Foamed Gelatin Polymer

This study deals with the effect of gelatin on physical and mechanical properties of  calcium phosphate bone cements. The mixture of tetracalcium phosphate (TTCP) and dicalcium phosphate (DCPA) as the cement powder was mixed with 6 wt% Na2HPO4 solution containing different amount (0, 2, 5 and 8% in w/w) of foamed gelatin as liquid phase. The physical properties were determined in the terms of s...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Dental materials journal

دوره 21 4  شماره 

صفحات  -

تاریخ انتشار 2002